The sweet, starchy orange sweet potatoes are delicious and nutritious ingredients for fries, casseroles, and pies. Although people have been growing sweet potatoes for 1000’s of years, scientists still do not know a lot about the protein make-up of these tubers. In ACS’ Journal of Proteome Analysis, scientists have investigated the proteome of sweet potato skins and roots, and during the process, have reported new insights into the plant’s genome.
The sweet potato (Ipomoea batatas, Lam.) is a staple food in some parts of the world, along with being used for animal feed and industrial items, akin to biofuels. The plant has a surprisingly complicated genome, encoding more expected genes than the human genome. Sweet potato further has a posh chemical composition, with low protein content in the roots and lots of secondary metabolites in the petals, making it tough to extract ample quantities of proteins for analysis.
Sorina and George Popescu and colleagues wanted to see whether or not a “proteogenomics” approach—analyzing both protein and genetic data collectively—could assist them in acquiring a better understanding of the structures of sweet potato roots and leaves.
The crew extracted proteins from root and leaf samples utilizing two different strategies and reduced them into peptides, which they analyzed with liquid chromatography and mass spectrometry. The researchers recognized 3,143 distinctive proteins from sweet potato leaves and 2,928 from roots.
Once they compared the proteomic information with the genome of the sweet potato, they recognized some regions within the revealed genome sequence where their data may provide improved data. For instance, the evaluation predicted 741 new protein-coding areas that previously weren’t considered genes. The group says the outcomes might be used to assist additional characterize and biofortify the tuber.